Application
Human Stomach stained with anti-Caspase-8 antibody| Product Name | Caspase 8 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 8 predilutedCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. |
| Immunogen | Recombinant full length human caspase 8 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE |
|---|---|
| Target | Caspase 8 |
| Cellular Localization | Cytoplasmic |
Application
Human Stomach stained with anti-Caspase-8 antibody| Positive Control | Jurkat cells, Stomach |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
