Application
IHC-P analysis of human breast lobule tissue using Cathepsin D antibody at a dilution of 1:50.Application
WB analysis of HepG2, Hep3B, MDA-MB-231 and MCF7 lysates (20ug per lane) using Cathepsin D antibody at a dilution of 1:1,000.| Product Name | Cathepsin D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4G2] to Cathepsin DThis gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human Cathepsin D (21-412 aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4G2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLN10 cathepsin D CTSD CPSD |
|---|---|
| Target | Cathepsin D |
| Light Chain Type | kappa |
Application
IHC-P analysis of human breast lobule tissue using Cathepsin D antibody at a dilution of 1:50.
Application
WB analysis of HepG2, Hep3B, MDA-MB-231 and MCF7 lysates (20ug per lane) using Cathepsin D antibody at a dilution of 1:1,000.| Application Notes | The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
