Application
MCF7 Human breast carcinoma cells were separated on SDS-PAGE and probed with Monoclonal AB to Cathepsin D (MO) CL:CTD-19 (Prepack) (Cat. No. ). The antibody was developed with AlkPhos APA mouse Fab ads HIgG conjugate and a NBT/BCIP substrate. Antibody dilution 1/4,000| Product Name | Cathepsin D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CTD - 19] to Cathepsin DCathepsin D has been reported to be involved in malignant tumor progression and with prognosis of various human cancers. |
| Immunogen | Full length native protein (purified) (Human liver). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | CTD-19 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PWB |
| For ELISA: Use at an assay dependant dilution. For IHC-P: Use at a dilution of 1:200. For WB: Use at a dilution of 1:1000. (antigen 34kD and a weaker band at 52kD). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTSD Cathepsin D 116840 MGC2311 P07339 CPSD 1509 |
|---|---|
| Target | Cathepsin D |
| Tissue Specificity | Recognizes cathepsin D (34 kDa and weaker band at 52 kDa) in immunoblotting. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H. |
Application
MCF7 Human breast carcinoma cells were separated on SDS-PAGE and probed with Monoclonal AB to Cathepsin D (MO) CL:CTD-19 (Prepack) (Cat. No. ). The antibody was developed with AlkPhos APA mouse Fab ads HIgG conjugate and a NBT/BCIP substrate. Antibody dilution 1/4,000| Positive Control | Human breast carcinoma tissue or cell line extract |
|---|---|
| Application Notes | For ELISA: Use at an assay dependant dilution. For IHC-P: Use at a dilution of 1:200. For WB: Use at a dilution of 1:1000. (antigen 34kD and a weaker band at 52kD). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 15mM sodium azide |
