Application
The recombinant fusion proteins (Cathepsin B, D, k, L and S), and cell lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cathepsin L (1:1000). Proteins were visualized using a goat anti-mouse secondary| Product Name | Cathepsin L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H7 ] to Cathepsin LCathepsin L, a lysosomal endopeptidase expressed in most eukaryotic cells, is a member of the papain-like family of cysteine proteinases. Cathepsin L can be induced by many different signaling events, including growth factor, secondary messenger and tumor promoters. Cathepsin L is translated as pre-procathepsin L, transferred through the Golgi apparatus as procathepsin L and then stored in lysosomes as mature cathepsin L. |
| Immunogen | Recombinant human Cathepsin L (114-333aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2H7 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Major excreted protein cathepsin L CATL FLJ31037 cathepsin L1 CTSL cathepsin L1 preproprotein |
|---|---|
| Target | Cathepsin L |
| Light Chain Type | kappa |
Application
The recombinant fusion proteins (Cathepsin B, D, k, L and S), and cell lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cathepsin L (1:1000). Proteins were visualized using a goat anti-mouse secondary| Application Notes | Western Blot: Use at 1:1,000 ~2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
