Application
Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L (Product No. C ). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate. Lane A: Antibody dilution 1:400 B: Negative control (only secondary antibody)| Product Name | Cathepsin L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [33/2] to Cathepsin LCathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intra-lysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive pro-form, and mRNA expression level seems to correlate with their metastatic potential. |
| Immunogen | Full length native protein (purified) (Human lung cancer line EPLC 32 M1) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 33/2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FWB |
| ELISA: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1:200 - 1:1000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatMink |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CATL CTSL FLJ31037 MEP |
|---|---|
| Target | Cathepsin L |
| Tissue Specificity | Reacts specifically with the native and denatured forms of human cathepsin L (25kD) and procathepsin L (42 kD). Does not react with human cathepsin types B1, D1, H and S1, procathepsins B and D1 and recombinant procathepsin H, rat cathepsin B1, and mouse procathepsins B1 and D1. |
| Cellular Localization | lysosomes |
Application
Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L (Product No. C ). The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate. Lane A: Antibody dilution 1:400 B: Negative control (only secondary antibody)| Positive Control | Whole cell extract from cultured mink lung cell line |
|---|---|
| Application Notes | ELISA: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1:200 - 1:1000. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 15mM sodium azide |
