Application
Anti-CaVgamma5_(extracellular) - Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1, 2. Anti-CaVγ5 (extracellular) antibody, (1:1000). 3, 4. Anti-CaVγ5 (extracellular) antibody preincubated with the control peptide antigen.Application
Anti-CaVgamma5_(extracellular) - Expression of CaVgamma5 in living rat PC12 cells Immunocytochemical staining of intact living rat PC12 cells using Anti-CaVγ5 (extracellular) antibody, (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue).| Product Name | Cav gamma 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cav gamma 5member of a family of gamma subunits of voltage-dependent calcium channels [RGD, Feb 2006] |
| Immunogen | Peptide (C)SESTVNVLKMIRS, corresponding to amino acid residues 87-99 of rat Cavγ5 (Accession Q8VHW8). 1st extracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cacng5 calcium channel voltage-dependent gamma subunit 5 |
|---|---|
| Target | Cav gamma 5 |
Application
Anti-CaVgamma5_(extracellular) - Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1, 2. Anti-CaVγ5 (extracellular) antibody, (1:1000). 3, 4. Anti-CaVγ5 (extracellular) antibody preincubated with the control peptide antigen.
Application
Anti-CaVgamma5_(extracellular) - Expression of CaVgamma5 in living rat PC12 cells Immunocytochemical staining of intact living rat PC12 cells using Anti-CaVγ5 (extracellular) antibody, (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue).| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
