Application
WB analysis of rat brain lysates using Cav2.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.Application
IHC analysis of mouse cerebellum using Cav2.1 antibody at a dilution of 1:100 (red) and GFAP antibody (green). (C) Merged image of panels A and B. Mol: molecular layer.| Product Name | Cav2.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cav2.1Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been |
| Immunogen | Peptide (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CaV2.1 (Accession P54282). Intracellular loop between domains II and III. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MHP SCA6 BI EA2 MHP1 HPCA FHM CACNL1A4 CAV2.1 calcium channel voltage-dependent P/Q type alpha 1A subunit CACNA1A APCA |
|---|---|
| Target | Cav2.1 |
Application
WB analysis of rat brain lysates using Cav2.1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.
Application
IHC analysis of mouse cerebellum using Cav2.1 antibody at a dilution of 1:100 (red) and GFAP antibody (green). (C) Merged image of panels A and B. Mol: molecular layer.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
