Application
WB analysis of rat brain membranes using Cav2.2 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.Application
IHC analysis of mouse cerebellum using Cav2.2 antibody at a dilution of 1:100 (red) and Calbindin 28K antibody (green). (C) Merged image of panels A and B. Mol: molecular layer.Application
ICC/IF analysis of rat DRG primary culture using Cav2.2 antibody at a dilution of 1:200 (red) and Hoechst 33342 (blue). (C) Merged images of panels A and B.| Product Name | Cav2.2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cav2.2 |
| Immunogen | Peptide (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat Cav2.2 (Accession Q02294). Intracellular loop between domains II and III. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cacna1b calcium channel voltage-dependent N type alpha 1B subunit |
|---|---|
| Target | Cav2.2 |
Application
WB analysis of rat brain membranes using Cav2.2 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.
Application
IHC analysis of mouse cerebellum using Cav2.2 antibody at a dilution of 1:100 (red) and Calbindin 28K antibody (green). (C) Merged image of panels A and B. Mol: molecular layer.
Application
ICC/IF analysis of rat DRG primary culture using Cav2.2 antibody at a dilution of 1:200 (red) and Hoechst 33342 (blue). (C) Merged images of panels A and B.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
