Application
CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 μg CBS antibody 7.5 % SDS-PAGE The immunoprecipitated CBS protein was detected by CBS antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
CBS antibody detects CBS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CBS protein stained by CBS antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS antibody at 1:500 dilution.Application
CBS antibody detects CBS protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CBS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CBS (cystathionine-beta-synthase)The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, BovineRat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIP4 antibody CBS antibody serine sulfhydrase antibody beta-thionase antibody cystathionine beta-synthase antibody methylcysteine synthase antibody cystathionine-beta-synthase antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 μg CBS antibody 7.5 % SDS-PAGE The immunoprecipitated CBS protein was detected by CBS antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
CBS antibody detects CBS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CBS protein stained by CBS antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS antibody at 1:500 dilution.
Application
CBS antibody detects CBS protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
