Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] diluted at 1:500.Application
CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS antibody [GT519] diluted at 1:250.Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:2000Application
CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. CBS antibody [GT519] diluted at 1:250.Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 µg rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:1000Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 30 µg Raji whole cell lysate/extract B. 30 µg K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:1000| Product Name | CBS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to CBS (cystathionine-beta-synthase)The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT519 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rabbit, BovineXenopus laevis (93%), Rabbit (95%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIP4 antibody CBS antibody serine sulfhydrase antibody beta-thionase antibody cystathionine beta-synthase antibody methylcysteine synthase antibody cystathionine-beta-synthase antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] diluted at 1:500.
Application
CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS antibody [GT519] diluted at 1:250.
Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:2000
Application
CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. CBS antibody [GT519] diluted at 1:250.
Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 µg rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:1000
Application
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 30 µg Raji whole cell lysate/extract B. 30 µg K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] dilution: 1:1000| Positive Control | 293T , Raji , K562 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
