Application
Surface staining of human peripheral blood cells with anti-CD11b (MEM-170) Alexa Fluor® 700.| Product Name | CD11b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MEM-170] to CD11bCD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages.CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.Entrez Gene: 16p11.2 |
| Immunogen | Human granulocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-170 |
| Host Species | Mouse |
| Tested Applications | FACSFunctional Assay |
| Functional: Blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD11B CR3A MAC-1 MAC1A MGC117044 MO1A Mac-1 alpha Mac1 alpha |
|---|---|
| Target | CD11b |
| Tissue Specificity | The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen. |
Application
Surface staining of human peripheral blood cells with anti-CD11b (MEM-170) Alexa Fluor® 700.| Application Notes | Functional: Blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. |
