Application
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 antibody [C1C3] dilution: 1:500.Application
CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 antibody [C1C3] dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:1000Application
CD146 antibody [C1C3] detects CD146 protein by western blot analysis. A. 50 μg rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000| Product Name | CD146 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD146 (melanoma cell adhesion molecule)Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD146 antibody MUC18 antibody MCAM antibody melanoma adhesion molecule antibody Gicerin antibody melanoma-associated antigen A32 antibody cell surface glycoprotein MUC18 antibody cell surface glycoprotein P1H12 antibody S-endo 1 endothelial-associa |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 antibody [C1C3] dilution: 1:500.
Application
CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 antibody [C1C3] dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:1000
Application
CD146 antibody [C1C3] detects CD146 protein by western blot analysis. A. 50 μg rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000| Positive Control | HeLa , HCT116 , Rat liver |
|---|---|
| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-p:1:50~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
