Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CD146 antibody at 1:500 dilution.Application
CD146 antibody detects CD146 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CD146 antibody diluted by 1:1000| Product Name | CD146 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD146 (melanoma cell adhesion molecule)Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CD146. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD146 antibody MUC18 antibody MCAM antibody melanoma adhesion molecule antibody Gicerin antibody melanoma-associated antigen A32 antibody cell surface glycoprotein MUC18 antibody cell surface glycoprotein P1H12 antibody S-endo 1 endothelial-associa |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CD146 antibody at 1:500 dilution.
Application
CD146 antibody detects CD146 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CD146 antibody diluted by 1:1000| Positive Control | A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
