| Product Name | CD146 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [P1H12] to CD146CD146 (also known as A32, MCAM, Mel-CAM, MUC18, and S-Endo-1) belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell-cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N-linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate-3-glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146. |
| Immunogen | Tissue / cell preparation (Human umbilical cord cells). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | P1H12 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIP |
| ELISA: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. ICC: Use at a dilution of 1/100. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 155735 MCAM CD146 4162 P43121 MUC18 |
|---|---|
| Target | CD146 |
| Tissue Specificity | Monoclonal Anti Endothelial cells (CD146) recognizes cultured microvascular and large vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation. |
| Positive Control | Frozen, acetone fixed sections of human tongue |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. ICC: Use at a dilution of 1/100. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | store in -20 C |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
