| Product Name | CD15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [28] to CD15The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri saccharide 3 fucosyllactosamine (3Fl) which is present in lacto N fucopentaose III and in the blood group antigen X hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. |
| Immunogen | Cell preparation: Monocytes, separated from other peripheral blood leucocytes (PBL) on fibronectin plates. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 28 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIP |
| FACS: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Note: samples should be acetone fixed. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | FUC-TIV 2526 ELFT FCT3A FUCTIV CD15 FUT4 P22083 104230 FUTIV LeX SSEA-1 SSEA 1 FUC TIV SSEA1 |
|---|---|
| Target | CD15 |
| Tissue Specificity | Specific to CD15. 28 is useful for leukaemia typing and purification of stem cells. |
| Cellular Localization | Type II membrane protein. Membrane bound form in trans cisternae of Golgi. |
| Application Notes | FACS: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Note: samples should be acetone fixed. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS |
