Application
Surface staining of human peripheral blood cells with anti-human CD15: FITC . Cells in the granulocyte gate were used for analysis| Product Name | CD15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-158] to CD15Monoclonal Anti-Human CD15 antibody recognizes the CD15 antigen. The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30-60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri-saccharide 3-fucosyllactosamine (3-Fl) which is present in lacto-N-fucopentaose III and in the blood group antigen X-hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. |
| Immunogen | Tissue / cell preparation (Human granulocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | MEM-158 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | SSEA1 FUC-TIV 2526 ELFT FCT3A FUCTIV CD15 FUT4 P22083 104230 FUC TIV |
|---|---|
| Target | CD15 |
| Tissue Specificity | Human CD15 antigen. |
| Cellular Localization | Cell Membrane |
Application
Surface staining of human peripheral blood cells with anti-human CD15: FITC . Cells in the granulocyte gate were used for analysis| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8ºC. Do not freeze. Do not use after expiration date stamped on vial label. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide,pH 7.4 |
