| Product Name | CD15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MY-1] to CD15Monoclonal Anti-Human CD15 antibody recognizes the CD15 antigen. The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30-60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri-saccharide 3-fucosyllactosamine (3-Fl) which is present in lacto-N-fucopentaose III and in the blood group antigen X-hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. |
| Immunogen | Purified neutrophils from normal human peripheral blood. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | MY-1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60 mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SSEA1 FUC-TIV 2526 ELFT FCT3A FUCTIV CD15 FUT4 P22083 104230 FUC TIV |
|---|---|
| Target | CD15 |
| Tissue Specificity | The specificity of this antibody is similar to antibodies Leu-M1, T‹ 9 and VIM-D5. It reacts with an antigen located on mature granulocytes and on Hodgkin and Reed Sternberg cells. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
| Positive Control | Tonsil. |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60 mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: Tissue culture supernatant |
