Mouse monoclonal [2H7] to human CD16 sampleThe human CD16 cell surface antigen is expressed by granulocytes, NK cells, macrophages and a subset of T-cells.
Immunogen
Recombinant human CD16
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2H7
Host Species
Mouse
Tested Applications
IHC-P
For IHC-P: Use at a dilution of 1:20-1:40. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 0.01M Sodium citrate buffer pH6.0 is recommended for this purpose. Not suitable for frozen sections. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CD16 FCG3 CD16a FCGR3 IGFR3
Target
CD16
Tissue Specificity
Recognizes the human CD16 cell surface antigen expressed by granulocytes, NK cells, macrophages and a subset of T-cells.
Application
Application
IHC-P ImageIHC-P analysis of human lung tissue using CD16 antibody [2H7] following heat mediated antigen retrieval using citrate buffer, pH6.0.
Positive Control
Tonsil
Application Notes
For IHC-P: Use at a dilution of 1:20-1:40. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 0.01M Sodium citrate buffer pH6.0 is recommended for this purpose. Not suitable for frozen sections. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.