Application
Surface staining of human peripheral blood cells with anti-human CD16 : PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects| Product Name | CD16 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-154] to CD16CD16 is a 50-65kD cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. |
| Immunogen | Tissue / cell preparation (Human granulocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-154 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Use under heat-denatured non-reducing conditions (no reducing agent). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD16a 2214 FCG3 FCGR3A CD16 146740 FCGR3 P08637 IGFR3 |
|---|---|
| Target | CD16 |
| Tissue Specificity | This antibody recognizes a polymorphism of human CD16a (NK cell form) and reacts well with the granulocyte form (CD16b) of all subjects. This antibody reacts with an epitope residing in proximity to FG loop (probably BC or C?E loop). |
Application
Surface staining of human peripheral blood cells with anti-human CD16 : PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects| Application Notes | FACS: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Use under heat-denatured non-reducing conditions (no reducing agent). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
