Mouse monoclonal antibody [BY55] to CD160CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MH
Key Feature
Clonality
Monoclonal
Isotype
IgM
Clone Number
BY55
Host Species
Mouse
Tested Applications
FACSIP
Flow Cytometry: 1/10 - 1/50 Optimal dilution / concentration should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
NK1 BY55 NK28
Target
CD160
Tissue Specificity
This antibody recognizes human CD160.
Application
Application
FACS analysis of human peripheral blood lymphocytes using CD160 antibody [BY55] (Azide free).
Application Notes
Flow Cytometry: 1/10 - 1/50 Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.