| Product Name | CD162 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3E2.25.5(PL1)] to CD162Selectin P ligand, or P-selectin glycoprotein ligand (PSGL-1), is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. |
| Immunogen | Human neutrophils. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3E2.25.5(PL1) |
| Host Species | Mouse |
| Tested Applications | BlockingFACSIHCIHC-FIHC-PWB |
| FACS: Use at an assay dependent dilution. IHC-Fr: 1/200. IHC-P: 1/200. WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | Q14242 6404 PSGL-1 CD162 600738 SELPLG PSGL1 PSGL 1 |
|---|---|
| Target | CD162 |
| Tissue Specificity | In peripheral blood, reacts with monocytes, granulocytes, T cells and a subset of B cells. The antibody does not react with erythrocytes and resting or thrombin activated platelets. is a blocking antibody since it inhibits the binding of P-selectin to PSGL-1 in a variety of assays. |
| Cellular Localization | Type I membrane protein |
| Application Notes | FACS: Use at an assay dependent dilution. IHC-Fr: 1/200. IHC-P: 1/200. WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS, 1mg/ml BSA |
