Application
Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).| Product Name | CD162 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [TC2] to CD162CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors. Entrez Gene: 12q24 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TC2 |
| Host Species | Mouse |
| Tested Applications | FACS |
| FACS: Use at 1.5 - 2.5 µg/mL. Positive Control:human peripheral blood: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD162 PSGL-1 PSGL1 |
|---|---|
| Target | CD162 |
| Tissue Specificity | The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34 + bone marrow cells. |
Application
Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).| Application Notes | FACS: Use at 1.5 - 2.5 µg/mL. Positive Control:human peripheral blood: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
