Application
Western Blot analysis of PMA-activated monocytes (Fig. 1B), using anti-human CD18 (MEM-148) . The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).Application
Western Blot analysis of PMA-activated neutrophils (Fig. 1A), using anti-human CD18 (MEM-148) . The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).| Product Name | CD18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 148] to CD18The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Tissue / cell preparation (Human). (Peripheral blood mononuclear cells). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-148 |
| Host Species | Mouse |
| Tested Applications | FACSIPWBFunctional Assay |
| FACS: Use at an assay dependant concentration. The antibody is excellent marker of activated myeloid cells. FuncS: Use at an assay dependant dilution. The antibody induces homotypic cell aggregation and high-affinity LFA-1 conformation inresting leukocytes. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 116920 LFA-1 P05107 3689 MF17 LFA1 ITGB2 LCAMB MFI7 LAD CD18 LFA 1 |
|---|---|
| Target | CD18 |
| Tissue Specificity | Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. |
Application
Western Blot analysis of PMA-activated monocytes (Fig. 1B), using anti-human CD18 (MEM-148) . The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).
Application
Western Blot analysis of PMA-activated neutrophils (Fig. 1A), using anti-human CD18 (MEM-148) . The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).| Application Notes | FACS: Use at an assay dependant concentration. The antibody is excellent marker of activated myeloid cells. FuncS: Use at an assay dependant dilution. The antibody induces homotypic cell aggregation and high-affinity LFA-1 conformation inresting leukocytes. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
