Application
Western Blot analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 . Lane 1: KG-1a human acute myeloid leukemia cell line Lane 2: HPB-ALL human peripheral blood T cell leukemia cell lineApplication
Surface staining of human peripheral blood cells with anti-human CD18: PE . Cells in the granulocyte gate were used for analysis.Application
Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 .| Product Name | CD18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-48] to CD18The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Tissue / cell preparation (Human). (Leucocytes of a patient suffering from a LGL-type leukaemia). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-48 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSIHC-FIHC-PIPWB |
| FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. The antibody induces high-affinity conformation of LFA-1 complex. IHC-Fr: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 116920 LFA-1 P05107 3689 MF17 LFA1 ITGB2 LCAMB MFI7 LAD CD18 LFA 1 |
|---|---|
| Target | CD18 |
| Tissue Specificity | Human CD18 antigen. This antibody recognizes all three leucocyte integrins. |
Application
Western Blot analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 . Lane 1: KG-1a human acute myeloid leukemia cell line Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line
Application
Surface staining of human peripheral blood cells with anti-human CD18: PE . Cells in the granulocyte gate were used for analysis.
Application
Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 .| Application Notes | FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. The antibody induces high-affinity conformation of LFA-1 complex. IHC-Fr: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
