Mouse monoclonal [L26] to CD20 - sampleThe CD20 antigen is present on human pre-B lymphocytes and on B-lymphocytes atall stage of maturation, except on plasma cells. Low level expression of the CD20antigen has been detected on normal T lymphocytes. CD20 is a non- glycosylated protein with a molecular weight of 35 or 37kDa depending on the degree of phosphorylation.
Immunogen
Human tonsil B cells
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
L26
Host Species
Mouse
Tested Applications
IHC-P
IHC:Use at 1:10-1:40 in an ABC method. This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.:
This antibody is specific to a 33 kDa polypeptide present on the majority of B cells in peripheral blood and lymphoid tissue and also with a minor component of 30 kDa. No reactivity with other haematopoietic cells has been observed.
Cellular Localization
Cell membrane and cytoplasm.
Light Chain Type
kappa
Application
Application
Formalin fixed paraffin embedded human tonsil stained with CD20, B cell antibody (cat. # ).
Application
- immunohistochemistry: Formalin fixed paraffin embedded human tonsil stained with CD20, B cell using ABC and Fast Red chromagen.
Positive Control
Tonsil
Application Notes
IHC:Use at 1:10-1:40 in an ABC method. This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.:
Additional Information
Form
Liquid
Storage Instructions
Store at 4-8 °C. Aliquot into suitable working units to avoid contamination.
Storage Buffer
Phosphate-buffered saline, pH 7.2, contaning 1%BSA and 0.09% sodium azide