Application
IHC-P analysis of rat spleen tissue using CD3 antibody [1F4].| Product Name | CD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F4] to CD3CD3 is a subunit of the TCR complex. It is involved in TCR assemby and signaling. |
| Immunogen | F344 rat T cells stimulated with PMA (TPA) and calcium ionophore |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 1F4 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-PIPWB |
| Flow Cytometry: 1/10 - 1/25: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | CD3 |
|---|---|
| Tissue Specificity | recognises rat CD3, a 25kD antigen, which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA. |
Application
IHC-P analysis of rat spleen tissue using CD3 antibody [1F4].| Application Notes | Flow Cytometry: 1/10 - 1/25: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
