Application
IHC Image using - Detection of Ki1 by IHC in Human Hodgkins Lymphoma| Product Name | CD30 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Ki - 1 Antigen CD30This antibody reacts with a 105 kD glycoprotein expressed by Hodgkin's and Reed-Sternberg cells and by tumors cells of majority of large cell lymphomas. In normal lymphoid tissues, it reacts with a small population of large cells present around Bcell follicles and some times at the rim of germinal centers. |
| Immunogen | BALB/C mice were immunized with L428 cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Ber-H2 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cd30 D1S166E Ki Ki-1 Ki 1 Ki1 |
|---|---|
| Target | Ki1 |
| Cellular Localization | Cell membrane |
| Light Chain Type | Kappa |
Application
IHC Image using - Detection of Ki1 by IHC in Human Hodgkins Lymphoma| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA buffer, pH 8.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containg 1% BSA and 0.09% sodium azide |
