Application
ICC/IF ImageA cluster of DAPI-stained rat vascular endothelial cells that were immunoreactive for CD31/PECAM (red) and the anandamide hydrolyzing enzyme, fatty acid amide hydrolase (FAAH) (green). Enzymatically-isolated rat vascular endothelial cells were incubated fApplication
Immunohistochemical analysis of paraffin-embedded human kidneys using anti-CD31. Note positive staining of endothelial cellsApplication
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:BiotinApplication
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:RPEApplication
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:FITCApplication
FACS ImageFACS profile of endothelial cells stained with anti-CD31 antibody. Solid profiles represent IgG isotype control. This image was kindly supplied as part of the review submitted by one of our customers.| Product Name | CD31 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [WM59] to CD31The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | WM59 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PIP |
| IP:1:100~1:500 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 IHC-f:1:100~1:500 FACS:1:200~1:500 |
|
| Species Reactivity | ChickenCynomolgus MonkeyHumanMouseRhesus Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | platelet/endothelial cell adhesion molecule 1 GPIIA' PECAM-1 PECAM1 endoCAM PECA1 CD31/EndoCAM |
|---|---|
| Target | CD31 |
| Tissue Specificity | This antibody recognizes the human CD31 antigen. Clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995). |
Application
ICC/IF ImageA cluster of DAPI-stained rat vascular endothelial cells that were immunoreactive for CD31/PECAM (red) and the anandamide hydrolyzing enzyme, fatty acid amide hydrolase (FAAH) (green). Enzymatically-isolated rat vascular endothelial cells were incubated f
Application
Immunohistochemical analysis of paraffin-embedded human kidneys using anti-CD31. Note positive staining of endothelial cells
Application
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:Biotin
Application
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:RPE
Application
FACS ImageStaining of human peripheral blood platelets with Mouse anti Human CD31:FITC
Application
FACS ImageFACS profile of endothelial cells stained with anti-CD31 antibody. Solid profiles represent IgG isotype control. This image was kindly supplied as part of the review submitted by one of our customers.| Application Notes | IP:1:100~1:500 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 IHC-f:1:100~1:500 FACS:1:200~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
