Application
FACS ImageStaining of human peripheral blood granulocytes with Mouse anti-Human CD32:Azide Free| Product Name | CD32 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AT10] to CD32 (azide free)This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | K562 cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT10 |
| Host Species | Mouse |
| Tested Applications | BlockingFACSIHC-FIPNeutFunctional Assay |
| For FACS: Use at a concentration of 20 μg/ml. Use 10 μl of the suggested working dilution to label 10^6 cells or 100 μl whole blood. For IHC-Fr: Use at a concentration of 20 μg/ml. For IP: Use at a concentration of 20 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDw32 2213 FcGR 604590 IGFR2 P12318 P31994 FCGR2A1 FCGR2B CD32 FCGR2A 2212 FCG2 FCGR2 MGC30032 MGC23887 146790 |
|---|---|
| Target | CD32 |
| Tissue Specificity | Recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. C D32 is expressed by B cells, monocytes, granulocytes and platelets. |
Application
FACS ImageStaining of human peripheral blood granulocytes with Mouse anti-Human CD32:Azide Free| Positive Control | Lymph Node |
|---|---|
| Application Notes | For FACS: Use at a concentration of 20 μg/ml. Use 10 μl of the suggested working dilution to label 10^6 cells or 100 μl whole blood. For IHC-Fr: Use at a concentration of 20 μg/ml. For IP: Use at a concentration of 20 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains no preservatives |
