WB
Western blot analysis of CD32B on different lysates with Mouse anti-CD32B antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 µg/Lane), Lane 2: Raji cell lysate (10 µg/Lane), Lane 3: HL-60 cell lysate (10 µg/Lane), Lane 4: Human placenta tissue lysate (20 µg/Lane), Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPXIHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD32B antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Tissue: Breast cancer, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBS, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Product Name | CD32B Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human aa 1-250 / 310. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:1000 IHC:1:200-1:1000 IF-P:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FCGR2B |
|---|---|
| Gene Synonyms | CD32 FCG2 CD32B FCGR2 IGFR2 |
| Gene Full Name | Fc gamma receptor IIb |
| Gene Summary | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Alternative Names | CD32 antibody CD32 antigen antibody CD32B antibody CDw32 antibody Fc fragment of IgG low affinity II receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor antibody Fc fragment of IgG low affinity IIb receptor (CD32) antibody Fc fragment of IgG low affinity IIb receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor for antibody Fc gamma receptor IIB antibody CD32 antibody CD32 antigen antibody CD32B antibody CDw32 antibody Fc fragment of IgG low affinity II receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor antibody Fc fragment of IgG low affinity IIb receptor (CD32) antibody Fc fragment of IgG low affinity IIb receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor for antibody Fc gamma receptor IIB antibody Fc gamma RII antibody Fc gamma RIIB antibody Fc-gamma RII antibody Fc-gamma RII-b antibody Fc-gamma-RIIb antibody FCG2 antibody FCG2B_HUMAN antibody Fcgr2 antibody FCGR2B antibody FCRII antibody FcRII-b antibody IGFR2 antibody IgG Fc receptor II beta antibody IgG Fc receptor II-b antibody Low affinity immunoglobulin gamma Fc region receptor II antibody Low affinity immunoglobulin gamma Fc region receptor II-b antibody Ly-17 antibody Lym 1 antibody Lymphocyte antigen 17 antibody |
| Molecular Weight(MW) | 34 kDa(Observed band size: 39 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD32B on different lysates with Mouse anti-CD32B antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 µg/Lane), Lane 2: Raji cell lysate (10 µg/Lane), Lane 3: HL-60 cell lysate (10 µg/Lane), Lane 4: Human placenta tissue lysate (20 µg/Lane), Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD32B antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD32B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Tissue: Breast cancer, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBS, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Application Notes | WB:1:1000 IHC:1:200-1:1000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
