| Product Name | CD34 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BI-3C5] to CD34The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Immunogen | Synthetic peptide – Immunogen sequence information is commercially sensitive |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BI-3C5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FWB |
| For WB: Tested against recombinant protein only. IHC-Fr: Use at a dilution of 1:400. Expected to work on FACS at a dilution of 1:400 (10μl per million cells) but not yet tested in house. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cluster designation 34 Hematopoietic progenitor cell antigen CD34 HPCA1 antibody |
|---|---|
| Target | CD34 |
| Tissue Specificity | Reacts with class I epitope of CD34 of human origin, which is sensitive to neuramidase, glycoprotease and chymopapain |
| Cellular Localization | Type I membrane protein. |
| Application Notes | For WB: Tested against recombinant protein only. IHC-Fr: Use at a dilution of 1:400. Expected to work on FACS at a dilution of 1:400 (10μl per million cells) but not yet tested in house. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
