| Product Name | CD35 antibody [E11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human CD35 / CR1 ( Follicular Dendritic Cell Marker )This antibody is specific to a 210-220 kD protein, which is identified as the complement receptor 1 (CR1/CD35). This antibody recognizes as site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This antibody does not cross react with CR2. |
| Immunogen | BALB/C mice were injected with intact human monocytes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | E11 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 120620, 1378, P17927, C3BR, CD35, CR1, KN |
|---|---|
| Target | CD35 |
| Cellular Localization | Cell membrane |
| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
