Application
FACS analysis of human peripheral blood lymphocytes using CD35 antibody [E11] (Azide free).Application
FACS analysis of human peripheral blood monocytes using CD35 antibody [E11] (Azide free).| Product Name | CD35 antibody (Azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [E11] to CD35This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, ha |
| Immunogen | Human acute monocytic leukaemia cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | E11 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-P |
| Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
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| Species Reactivity | HumanBaboonRhesus MonkeyCynomolgus Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C4BR C3BR CR1 KN |
|---|---|
| Target | CD35 |
| Tissue Specificity | This antibody recognizes CD35. |
Application
FACS analysis of human peripheral blood lymphocytes using CD35 antibody [E11] (Azide free).
Application
FACS analysis of human peripheral blood monocytes using CD35 antibody [E11] (Azide free).| Application Notes | Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline |
