Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.Application
Flow cytometric analysis of live Jurkat cells, using anti-CD4 antibody ( at 1:100, Red), compared to IgG1 isotype control (Black).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.| Product Name | CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [18E3] to CD4Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Immunogen | Protein expressed in 293T cell transfected with human CD4 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 18E3 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD4mut |
|---|---|
| Target | CD4 |
| Tissue Specificity | is specific for human CD4. |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.
Application
Flow cytometric analysis of live Jurkat cells, using anti-CD4 antibody ( at 1:100, Red), compared to IgG1 isotype control (Black).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
