| Product Name | CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human CD4This antibody reacts specifically with CD4 cells. CD4 is a single chain transmembrane glycoprotein with a molecular weight of 59 kD. The CD4 antigen is found on a T cell subset (helper/inducer) and also on thymocytes and monocytes. |
| Immunogen | Synthetic peptide corresponding to the external domain of CD4 molecule. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F6 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking technique is required for paraffin embedded tissue sections. We recommend using 1mM EDTA pH 8.0 buffer instead of sodium citrate pH 6.0 buffer for antigen unmasking.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD4mut 920 P01730 CD4 186940 |
|---|---|
| Target | CD4 |
| Cellular Localization | Cell membrane |
| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature or overnight at 40 C. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking technique is required for paraffin embedded tissue sections. We recommend using 1mM EDTA pH 8.0 buffer instead of sodium citrate pH 6.0 buffer for antigen unmasking.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
