Application
Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-human CD4 (MEM-241). Lane 1: original lysate of T cells Lane 2: immunoprecipitate by negative control antibody Lane 3: immunoprecipitate by anti-human CD4 (MEM-115)| Product Name | CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to CD4 (Human)This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq] |
| Immunogen | Human thymocytes and T lymphocytes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-115 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| Immunoprecipitation: excellent, Functional Application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature monovalent Fab fragments essentially do not bind to T cells it is negative in Western blotting even with non-reduced samples of cell lysates.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 920 P01730 CD4 186940 CD4mut |
|---|---|
| Target | CD4 |
| Tissue Specificity | The antibody recognizes an epitope in the D1 domain of CD4 antigen, a transmembrane glycoprotein (59 kDa) of the immunoglobulin supergene family present on subset of T lymphocytes ("helper/inducer" T cells) and also expressed at a lower level on monocytes and granulocytes. Gene location: human chromosome 12p13.31 HLDA V.; WS CODE CD04.09 |
Application
Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-human CD4 (MEM-241). Lane 1: original lysate of T cells Lane 2: immunoprecipitate by negative control antibody Lane 3: immunoprecipitate by anti-human CD4 (MEM-115)| Application Notes | Immunoprecipitation: excellent, Functional Application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature monovalent Fab fragments essentially do not bind to T cells it is negative in Western blotting even with non-reduced samples of cell lysates.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
