| Product Name | CD4 antibody (azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [RPA-T4] to CD4 (azide free)CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. |
| Immunogen | Human PHA blasts |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RPA-T4 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| For FACS: Use at a dilution of 1:50 - 1:100. Use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood Method sheets are available on request. For IHC-Fr: Use at a dilution of 1:200 - 1:2,000. Not recommended for the use of IHC-P. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CD4 Antigen antibody CD4 molecule antibody CD4 Receptor antibody CD4mut antibody T Cell Antigen T4 antibody T Cell Surface Antigen T4/Leu3 antibody T Cell Surface Glycoprotein CD4 antibody |
|---|---|
| Target | CD4 |
| Tissue Specificity | Recognizes the human CD4 cell surface antigen, a 55kD glycoprotein expressed by the helper-inducer subset of T lymphocytes and more weakly by monocytes. |
| Positive Control | Human lymphatic tissue |
|---|---|
| Application Notes | For FACS: Use at a dilution of 1:50 - 1:100. Use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood Method sheets are available on request. For IHC-Fr: Use at a dilution of 1:200 - 1:2,000. Not recommended for the use of IHC-P. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline |
