Mouse monoclonal [W3/25] to rat CD4MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD]
Immunogen
Rat Thymocyte Membrane Preparation
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
W3/25
Host Species
Mouse
Tested Applications
FACSIHC-FIHC-P
For FACS: Use at a dilution of 1:100 - 1:200. For IHC-Fr and IHC-P: Use at an assay dependent dilution. For Functional studies: Use at an assay dependent solution. Optimal concentrations/dilutions should be determined by the reseacher.Immunohistology: *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde):
Species Reactivity
Rat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
W3/25 p55
Target
CD4
Tissue Specificity
recognises the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells, thymocytes and macrophages. This antibody inhibits proliferation and IL-2 production in the MLR reaction.
Application Notes
For FACS: Use at a dilution of 1:100 - 1:200. For IHC-Fr and IHC-P: Use at an assay dependent dilution. For Functional studies: Use at an assay dependent solution. Optimal concentrations/dilutions should be determined by the reseacher.Immunohistology: *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde):
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.