| Product Name | CD41a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [HIP8] to CD41aIntegrin alpha 2b (CD41) is a calcium-depedent, noncovalently associated heterodimer and contains a heavy chain (GPIIb-alpha) and a light chain (GPIIb-beta) linked by a single disulfied bond.T he integrin alpha 2B chain interacts with the integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major integrin on platelets and is important for platelet adhesion and aggregation. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HIP8 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| FACS: Tested on human platelets. This can be used at less than or equal to 0.5 mg per 100 ml blood (per 10 6 cells). IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | CD41 CD41B GP2B GPIIb GTA HPA3 |
|---|---|
| Target | CD41a |
| Tissue Specificity | reconizes the GPIIb-alpha (heavy chain) of integrin alpha 2B.The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. [HIP8] blocks platelet aggregation andcompletely inhibits ADP, epinephrine, and collagen-induced platelet activation, and partially inhibits ristocetin and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes. |
| Application Notes | FACS: Tested on human platelets. This can be used at less than or equal to 0.5 mg per 100 ml blood (per 10 6 cells). IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
