Application
FACS analysis of human peripheral blood platelets using CD42b antibody [AK2] (Low endotoxin, azide free).| Product Name | CD42b antibody (Azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AK2] to CD42bGlycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease. [provided by RefSeq, Mar 2010] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AK2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIPFunctional Assay |
| Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DBPLT3 GPIBA CD42B CD42B-ALPHA GP1B GP1BA VWDP BSS BDPLT3 BDPLT1 |
|---|---|
| Target | CD42b |
| Tissue Specificity | This antibody recognizes human CD42b. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets |
Application
FACS analysis of human peripheral blood platelets using CD42b antibody [AK2] (Low endotoxin, azide free).| Application Notes | Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline |
