Application
Staining of rat splenocytes with Mouse anti Rat CD43: FITC| Product Name | CD43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [W3-13 HLK] to CD43The CD43 antigen recognised is a heavily glycosylated glycoprotein of mw 95,000 which has a high content of O-linked carbohydrate structures. On thymocytes this glycoprotein is the main target for binding of peanut lectin (4). This antibody is useful for distinguishing B and T lymphocytes and also for analysis of NK cells (5) and stem cells (1). |
| Immunogen | Rat thymocyte membrane |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | W3/13 HLK |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-P |
| For FACS: Use at a dilution of 1:50 - 1:100. For IHC-Fr and IHC-P: Use at an assay dependent solution. Optimal concentration/dilutions should be determined by the researcher.Immunohistology: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BSN2 DKFZp686A01127 FLJ20043 FLJ34928 |
|---|---|
| Target | CD43 |
| Tissue Specificity | Rat CD43 |
Application
Staining of rat splenocytes with Mouse anti Rat CD43: FITC| Application Notes | For FACS: Use at a dilution of 1:50 - 1:100. For IHC-Fr and IHC-P: Use at an assay dependent solution. Optimal concentration/dilutions should be determined by the researcher.Immunohistology: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 0.02% sodium azide |
