Application
FACS ImageStaining of human peripheral blood lymphocytes with Mouse anti Human CD45RO| Product Name | CD45RO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [UCHL1] to human CD45ROCD45 is a member of the tyrosine phosphatase (PTP) family. B-cell and T-cell antigen receptor signaling is regulated by this protein. The CD45RO isoform is present on most thymocytes including a subset of resting CD4 and CD8 T-cells. CD45RO performs a key role in lymphocyte activation. |
| Immunogen | Human IL-2 dependent T cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | UCHL1 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-PIP |
| For FACS: Use at a dilution of 1:50. For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LCA LY5 B220 CD45 T200 GP180 |
|---|---|
| Target | CD45RO |
| Tissue Specificity | Recognises the low molecular weight isoform (180kD) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood, UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes. |
Application
FACS ImageStaining of human peripheral blood lymphocytes with Mouse anti Human CD45RO| Positive Control | Tonsil |
|---|---|
| Application Notes | For FACS: Use at a dilution of 1:50. For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH7.2 containing no preservatives |
