| Product Name | CD53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [63 - 5A3 ( Workshop V; Code CD53.1 )] to CD53CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T and B cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-hemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appears to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst. |
| Immunogen | Human Sezary cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 63-5A3 (Workshop V; Code CD53.1) |
| Host Species | Mouse |
| Tested Applications | FACSICC/IF |
| For FACS: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | P19397 151525 TSPAN25 963 MOX44 CD53 |
|---|---|
| Target | CD53 |
| Cellular Localization | Cell membrane. |
| Light Chain Type | kappa |
| Positive Control | Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Tonsil and lymph node. |
|---|---|
| Application Notes | For FACS: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
