Mouse monoclonal antibody [MEM-53] to CD53The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MEM-53
Host Species
Mouse
Tested Applications
FACSIHC-FIPWB
Flow Cytometry: 1/10 - 1/25 Optimal dilution / concentration should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
TSPAN25 CD53 molecule MOX44
Target
CD53
Tissue Specificity
This antibody recognizes human CD53 cell surface antigen.
Application
Application
FACS ImageStaining of human peripheral blood monocytes with Mouse anti Human CD53:FITC
Application
FACS ImageStaining of human peripheral blood granulocytes with Mouse anti-Human CD53
Application Notes
Flow Cytometry: 1/10 - 1/25 Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.