Application
CD59 antibody detects CD59 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. CD59 antibody diluted at 1:500.Application
CD59 antibody detects CD59 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD59 antibody diluted at 1:1000.| Product Name | CD59 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD59 (CD59 molecule, complement regulatory protein)This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD59. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MEM43 MIC11 EJ30 p18-20 MIN2 MSK21 16.3A5 MAC-IP HRF-20 EL32 MACIF MIN1 1F5 CD59 molecule complement regulatory protein EJ16 MIRL MIN3 G344 HRF20 |
|---|---|
| Target | CD59 |
| Molecular Weight(MW) | 14 kDa (note) (8) |
| Cellular Localization | Cell membrane , GPI-anchor , Secreted |
Application
CD59 antibody detects CD59 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. CD59 antibody diluted at 1:500.
Application
CD59 antibody detects CD59 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD59 antibody diluted at 1:1000.| Positive Control | K562 , HL-60 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
