Application
Surface staining of human peripheral blood cells with anti-CD66acde monoclonal antibody clone CLB-gran/10Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-CD66acde clone CLB-gran/10 .| Product Name | CD66acde antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [CLB-gran/10] to CD66acdeThe CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety. |
| Immunogen | Human granulocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CLB-gran/10 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD66e CEA DKFZp781M2392 |
|---|---|
| Target | CD66acde |
| Tissue Specificity | The mouse monoclonal antibody CLB-gran/10 (IH4Fc) detects human CD66acde antigen, a granulocyte marker, especially after cell stimulation. The antibody does not cross-react with normal human peripheral B cells, T cells, monocytes and platelets. Weak reactivity has been observed with malignant cells of pacients with B cell-derived CLL. In immunohistochemistry the antibody reacts with some tissue macrophages and carcinoma-expressed CEA. |
Application
Surface staining of human peripheral blood cells with anti-CD66acde monoclonal antibody clone CLB-gran/10
Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-CD66acde clone CLB-gran/10 .| Application Notes | Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
