Application
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30). Primary antibody dilution: 10 µg/ml.Application
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30) . Primary antibody dilution: 10 μg/ml.| Product Name | CD66e antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [CB30] to CD66eThe CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion.Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.Protein Reviews On The WebEntrez Gene: 19q13.1-q13.2 |
| Immunogen | Human carcinoembryonic antigen (CEA; CEACAM5) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CB30 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-PIP |
| IHC-P: Staining technique: Standard ABC technique (DAB+)Use at 10 µg/mL (1:100). Positive tissue: Adenocarcinoma of colonPretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD66e CEA DKFZp781M2392 |
|---|---|
| Target | CD66e |
| Tissue Specificity | The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells. |
Application
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30). Primary antibody dilution: 10 µg/ml.
Application
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30) . Primary antibody dilution: 10 μg/ml.| Application Notes | IHC-P: Staining technique: Standard ABC technique (DAB+)Use at 10 µg/mL (1:100). Positive tissue: Adenocarcinoma of colonPretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
