Application
Formalin fixed paraffin embedded human tonsil stained with CD68 antibody.| Product Name | CD68 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Macrophage CD68This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells. |
| Immunogen | Subcellular fraction of human alveolar macrophages. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | KP1 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| For IHC-P: Use at a dilution of 1:20-1:50 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | CD68 |
|---|---|
| Cellular Localization | Cell membrane |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human tonsil stained with CD68 antibody.| Positive Control | Tonsil |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:20-1:50 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
