Application
IHC-P analysis of human tonsil using CD68 antibody [PG-M1].Application
Formalin fixed paraffin embedded human tonsils stained with anti-Macrophage CD68 antibody .| Product Name | CD68 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PG-M1] to CD68 predilutedCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
| Immunogen | BALB/C mice were immunized with Gaucher’s cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | PG-M1 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. This antibody is not recommended for frozen sections or cell smears.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 153634 12514 gp110 Cd68 968 P34810 Scard1 |
|---|---|
| Target | CD68 |
| Tissue Specificity | This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral allgranulocytes are negative.This antibody does not react with granulocytes and their precursor cells. |
| Light Chain Type | kappa |
Application
IHC-P analysis of human tonsil using CD68 antibody [PG-M1].
Application
Formalin fixed paraffin embedded human tonsils stained with anti-Macrophage CD68 antibody .| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections. This antibody is not recommended for frozen sections or cell smears.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with sodium azide |
