| Product Name | CD71 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MEM-189] to CD71Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Immunogen | KG1 human acute myelogenous leukaemia cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-189 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB (non-reducing conditions): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | TFR TFR1 7037 190010 Transferrin receptor TFRC CD71 P02786 TRFR |
|---|---|
| Target | CD71 |
| Tissue Specificity | Reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. It does not block binding of transferrin to the receptor. |
| Application Notes | For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB (non-reducing conditions): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15mM sodium azide |
